NUCLEOTIDE SEQUENCES OF HUMAN GLOBIN MESSENGER RNA
نویسندگان
چکیده
منابع مشابه
Nucleotide sequences of human globin messenger RNA.
Globin messenger RNA, isolated from human peripheral blood reticulocytes, was transcribed into complementary DNA by use of the RNA-dependent DNA polymerase of avian myeloblastosis virus. The complementary DNA was then transcribed into (32)P-labeled complementary RNA by E. coli RNA polymerase in the presence of alpha-(32)P-labeled ribonucleoside triphosphates. The fingerprint pattern obtained fr...
متن کاملNucleotide sequences of the 3'-terminal untranslated region of messenger RNA for human beta globin chain.
In normal messenger RNA for the human beta-globin chain, nucleotide sequences have been identified which can be matched to the amino-acid sequence of the abnormally long segment of the beta-chain of hemoglobin Cranston. The finding of these sequences strengthens the hypothesis that the betaCranston chain arose by a frameshift mutation allowing the "readthrough" of the normal termination codon a...
متن کاملDemonstration of globin messenger sequences in giant nuclear precursors of messenger RNA of avian erythroblasts.
Highly purified globin mRNA from ducks was copied with RNA-directed DNA polymerase from avian myeloblastosis virus into anti-messenger DNA. With excess RNA, more than 90% of this DNA annealed back to its template with a C(o)t/2 value of 7.5 x 10(-4) mol.sec. liter(-1); the melting temperature of the hybrid was 86 degrees . Giant nuclear RNA fractions with sedimentation coefficients of more than...
متن کاملGlobin messenger RNA in hemoglobin H disease.
Functional messenger RNA (mRNA) for human globin synthesis was isolated from reticulocytes of each of two patients with hemoglobin H disease. The RNA was tested for its capacity to direct globin synthesis in a messenger RNA-dependent cell-free system derived from Krebs Type II mouse ascites tumor cells. In each case, hemoglobin H disease mRNA directed the synthesis of a great excess of /3-globi...
متن کاملEvidence of Limited Structural Organization in Globin Intron Sequences of Messenger RNA.
The structural significance of introns in alpha and beta globin genes were evaluated in terms of the folding (or stacking) free energy of the nascent RNA sequences as obtained from secondary structure calculations. From calculations of the folding free energy of exons and introns, it was found that introns tend to have a more folded (globular) structure compared to the exon counterparts segment...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Annals of the New York Academy of Sciences
سال: 1974
ISSN: 0077-8923,1749-6632
DOI: 10.1111/j.1749-6632.1974.tb21888.x